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| Id: | 18946
| | Autor: | Teles, Rosane M. B; Moraes, Milton O; Geraldo, Natasha T. R; Salles, Ana M; Sarno, Euzenir N; Sampaio, Elizabeth P
| | Título: | Differential TNFalpha mRNA regulation detected in the epidermis of leprosy patients
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| | Fonte: | s.l; s.n; 2002. 8 p. ilus, tab, graf.
| | Resumo: | The epidermis is an important site of the immunoinflammatory response in the skin. In the present study, the expression of cytokine and ICAM-1 (intercellular adhesion molecule-1) genes was evaluated by RT-PCR in the epidermis isolated from biopsies from 25 reactional leprosy patients. TNFalpha and IL-6 mRNAs were detected in all individuals during the reactional state (reversal reaction or erythema nodosum leprosum), IL-8 message was detected in 66.6 per cent and 62.5 per cent of the patients, IL-12 mRNA was present in 91.6 per cent and 62.5 per cent and ICAM-1 in 100 per cent and 71.4 per cent, respectively. In addition, when skin biopsies were obtained from the same patients before and during the reactional episode, an enhancement in cytokine mRNA, but not in ICAM-1 mRNA, was observed. Seven patients were also evaluated at the onset of reaction and during antiinflammatory treatment. In contrast to a preferential decrease in the TNFalpha gene detected in the dermis, during the treatment phase, persistent/enhanced TNFalpha mRNA expression was detected in the epidermis in six out of the seven patients assessed. This peculiar pattern of expression might reflect a differential impact that in vivo antiinflammatory therapy has on the epidermis. The present findings indicate that the epidermis plays an important role in the local inflammatory response in leprosy and that the profile of response detected in the epidermis during the reactions may be regulated differently from that in the dermis. (AU).
| | Descritores: | CITOCINAS/genet
CITOCINAS/metab
ANTIINFLAMATORIOS ESTERÓIDES/uso terap
DERME/metab
HANSENOSTATICOS/uso terap
EPIDERME/metab
MOLECULA 1 DE ADESAO INTERCELULAR/genet
MOLECULA 1 DE ADESAO INTERCELULAR/metab
HANSENIASE/quimioter
HANSENIASE/metab
PENTOXIFILINA/uso terap
ANTIINFLAMATORIOS/uso terap
RNA MENSAGEIRO/metab
TALIDOMIDA/uso terap
PREDNISONA/uso terap
FATOR DE NECROSE TUMORAL/genet
FATOR DE NECROSE TUMORAL/metab
| | Limites: | HUMANO
MASCULINO
FEMININO
ADULTO
MEIA-IDADE
IDOSO
SUPPORT, NON-U.S. GOV'T
| | Meio Eletrônico: | - .
| | Localização: | BR191.1; 09157/s |
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